Figure 6.

Diagram illustrating the region of IbWRKY2 involved in self-transcriptional activation activity and assay. (a) Schematic diagrams represent the IbWRKY2 fragments encoding different parts of IbWRKY2 that were cloned into the pGBKT7 vector. M1 is a C-terminal deletion mutant, M2 is an N-terminal deletion mutant, and M3 has both the C- and N-terminal regions deleted. The numbers denote IbWRKY2 amino acid positions. (b) Transactivation assay of IbWRKY2 and its deletion mutants. The transactivation activity is shown on the right (plus sign is positive and minus sign is negative).