Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 21;9(4):1031. doi: 10.3390/cells9041031

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Either low or high dose of NNK reduce MLH1 expression in both lung (NCI) and head and neck (FaDu) cancer cells. Immunofluorescence staining for MLH1 reveals that (A) NCI and (B) FaDU exposed to either 1 μM or 2 μM of NNK produce reduced MLH1 nuclear levels, as indicated by (A-a and B-a) the weak nuclear staining of MLH1 (green: MLH1; blue: DAPI for nuclear staining; scale bar: 20 μm), and (A-b and B-b) the significantly lower nuclear MLH1 levels [MLH1/DAPI means(SD) bin count, by Zen imaging software], compared to untreated controls. Data were obtained from four independent images (p values by t-test; multiple comparisons by Holm-Sidak; GraphPad Prism 7.0).