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Infection of cells by Z1-LENTIluc. The pseudotype Z1-LENTIluc was synthesized using the three plasmids pME-Z1, psPAX2, and pLenti-luciferase-P2A-Neo (Lenti-luc). Cell culture supernatants from HEK293T transfected cells were tested at 1:5 dilutions on cell layers grown in 96-well cell culture plates. Luciferase activity in target cells was measured at day 3 post infection. Pseudotypes with VSV-G envelope expressed by pMD2.G were used for comparison.
