Figure 2.

Effects induced by the 4D3 antibody on paclitaxel (PTX)‐induced antitumoral effect in human invasive ductal carcinoma cell lines. A, mRNA expressions of CLDN4, CLDN1 and estrogen receptor (ER) with or without estradiol (1 nM)43 in MDA‐468 and MCF‐7 cell lines. Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was examined as loading control. B, C, Effect of 4D3 (1 μg/mL) on growth inhibition by PTX (10 or 20 nM)44 in comparison with that of 2C1 (1 μg/mL) in MDA‐468 cells (B) and MCF‐7 cells (C). D, The intracellular PTX concentration was measured by ELISA in cells treated with PTX (20 nM)44 exposed to 4D3 (1 μg/mL) or none. (E) Induction of apoptosis by PTX (20 nM)44 with or without exposure to 4D3 (1 μg/mL). Error bar, standard deviation (SD) from three independent examinations