Figure 3.

The combined effects of paclitaxel (PTX) and 4D3 in vivo. A, Subcutaneous tumors of MDA‐468 and MCF‐7 cells in nude mice were treated with PTX (10 mg/kg BW)25 and/or 4D3 (1 mg/kg BW) on Days 1, 3 and 7. Tumor weights 4 weeks after inoculation are displayed. B, Lung metastasis resulted by caudal inoculation of MDA‐468 and MCF‐7 cells in nude mice that were treated with PTX (10 mg/kg BW)25 and/or 4D3 (1 mg/kg BW) on Days 1, 3 and 7. Lung weights, 4 weeks after inoculation, are displayed. C, mRNA expression of markers for mouse stromal cells in the subcutaneous tumors was examined by RT‐PCR. Batf2, M1 macrophage; Fizz, M2 macrophage; CD73, mesenchymal stem cell. D, mRNA expression of human stemness‐associated genes, CD133 and CD44, was examined by RT‐PCR. E, Intratumoral pH of the subcutaneous tumors was measured. F, Intracellular pH and extracellular pH were measured in spheres of MDA‐468 and MCF‐7 cells with or without 4D3 treatment. G, mRNA expression of human stemness‐associated genes was examined in invasive ductal carcinoma (IDC) cells cultured in media adjustment of the pH to 7.4 or 7.0. Error bar, standard deviation (SD) from five mice. β‐Actin (BACT) was examined as a loading control