Figure 1.

MicroRNA (miR)‐375‐3p is widely downregulated in human colorectal cancer and promotes the sensitivity of cells to 5‐fluorouracil (5‐FU). A, Expression levels of miR‐375‐3p were detected by quantitative real‐time (qRT)‐PCR in 30 pairs of tumor tissues and matched adjacent normal tissues. B, Expression levels of miR‐375‐3p in different CRC cells and in normal colon epithelium cell lines were detected by qRT‐PCR. C, Expression levels of miR‐375‐3p in HCT116 and HT29 cells treated with miR‐375‐3p mimic or miR‐NC (negative control) for 24 h were measured by qRT‐PCR analysis. D, E, HT29 (D) and HCT116 cells (E) treated with miR‐375‐3p mimic or miR‐NC were incubated with different concentrations of 5‐FU for 24 h, and 5‐FU chemosensitivity was determined by CCK‐8 assay. All data are shown as the means ± SEM of 3 independent experiments. *P < .05, **P < .01, ***P < .001, ****P < .0001