Figure 2.

MicroRNA (miR)‐375‐3p increased the sensitivity of cells to 5‐fluorouracil (5‐FU) in vitro by regulating malignant phenotypes. A, B, Treatment with miR‐375‐3p in combination with 5‐FU resulted in significantly lower viability of (A) HT29 and (B) HCT116 cells than did any other treatment. OD, optical density. C, Transwell invasion (with Matrigel) and migrations (no Matrigel) assays with HCT116 and HT29 cells subjected to different treatments (×200) (left). Histograms indicate the number of invading and migrating cells (right). D, Apoptosis of HT29 and HCT116 cells at 48 h after transfection was detected by annexin V/propidium iodide labeling and flow cytometry. Quadrants from the lower left (counterclockwise) represent healthy, early apoptotic, late apoptotic, and necrotic cells, respectively. Evaluation of apoptosis was based on the number of apoptotic cells relative to the total cell number. Cells were treated with 100 nmol/L miRNA and 1 μg/mL 5‐FU concentration. All data are shown as the means ± SEM of 3 independent experiments. *P < .05, **P < .01, ****P < .0001