Figure 5.

Restoration of microRNA (miR)‐375‐3p expression overcomes 5‐fluorouracil (5‐FU) resistance in vivo. A, Cellular uptake of miR‐375‐3p delivered by cationic liposomes in HT29 cells was visualized by fluorescence microscopy. Colorectal cancer cells were incubated with Cy3‐labeled miR‐375‐3p for 1.5 h, stained with DAPI, and observed under a fluorescence microscope (×100). B, Morphology of tumors excised from mice at the end of the experiment. C, Body weight of mice after they were killed. D, Growth curves of xenograft tumors. Results are presented as the means ± SEM of 4 animals per group. E‐G, Changes in renal and liver function after different drug treatments. H, Immunohistochemical analysis of the proliferation marker Ki‐67, thymidylate synthase (TYMS), and apoptotic proteins Bax and Bcl‐2 (×200). ALT, alanine transaminase; AST, aspartate transaminase; NP, nanoparticle