Figure 6.

Increased expression of Per1 inhibits the tumorigenesis of oral squamous cell carcinoma (OSCC) cells in vivo. A, The weights and volumes of tumors in the Per1‐OE group were significantly lower than those in the Per1‐NC group (N = 5). B, The growth curve from an in vivo tumorigenicity assay showed that subcutaneous tumor growth in the Per1‐OE group was significantly slower than that in the Per1‐NC group. C, Tumor‐forming tissues in vivo were assessed by RT‐qPCR. The mRNA expression levels of Per1, LC3B and BAX in the Per1‐OE group were significantly increased, while the mRNA expression levels of Ki67 were significantly decreased. D, Western blotting was used to detect protein expression in tumor‐forming tissues in vivo. The LC3BII/LC3BI ratio and protein expression of Per1 and BAX were significantly increased in the Per1‐OE group, while the protein expression of p‐AKT, p‐mTOR, P62 and Ki67 was significantly decreased. All data represent three independent experiments. The results are shown as the mean ± SD (n ≥ 3). *P < .05; **P < .01; ***P < .001; ****P < .0001