Figure 1.

CagA interacts with SH2 domain‐containing phosphatidylinositol 5′‐phosphatase 2 (SHIP2) in a tyrosine phosphorylation‐dependent manner. A, Schematic diagram of Flag‐tagged CagA constructs. B‐E, AGS cells (B, C) or GES‐1 cells (B) were transiently transfected with a Flag‐tagged WT‐CagA or phosphorylation‐resistant (PR)‐CagA vector. COS‐7 cells (D, E) were transiently transfected with the indicated Flag‐tagged CagA vector together with a Myc‐tagged SHIP2 or control empty vector. Total cell lysates (TCLs) were immunoprecipitated (IP) with an anti‐Flag Ab and were subjected to immunoblotting (IB) with the respective Abs. EPIYA, Glu‐Pro‐Ile‐Tyr‐Ala motif