Figure 2.

SH2 domain of SH2 domain‐containing phosphatidylinositol 5′‐phosphatase 2 (SHIP2) is essential for binding to CagA. A, Schematic diagram of Myc‐tagged SHIP2 constructs. B, COS‐7 cells were transiently transfected with a Myc‐tagged WT‐SHIP2 or mutant SHPI2 lacking the SH2 domain (ΔSH2‐SHIP2) vector together with a Flag‐tagged WT‐CagA or control empty vector. Total cell lysates (TCLs) were immunoprecipitated (IP) with an anti‐Flag Ab and subjected to immunoblotting (IB) with the respective Abs