Table 1.

Custom Dark Probe TaqMan® assays for DropPhase2D6.

TaqMan® assay ID	DropPhase2D6 assay name	Custom ID	rs number	SNP location	Dye	Fluorophore on	NFQ on
C__27102425_10	rs16947varT	C__27102425_10AF	rs16947	exon 6	FAM	A	G
C__27102425_10	rs16947refC	C__27102425_10GF	rs16947	exon 6	FAM	G	A
C__27102431_D0	rs3892097varT	C__27102431_D0TF	rs3892097	intron 3	FAM	C	T
C__61226317_10	rs28817600varG	C__61226317_10GV	rs28817600	25 kb	VIC	G	A
C__30485108_10	rs5758562varC	C__30485108_10CV	rs5758562	75 kb	VIC	C	G
C__29692254_10	rs5758550varG	C__29692254_10GV	rs5758550	116 kb	VIC	G	A
C__29692254_10	rs5758550refA	C__29692254_10AV	rs5758550	116 kb	VIC	A	G

TaqMan® Assay ID and Custom Assay ID refer to the original TaqMan® assays and their corresponding customized assay. The fluorescent dye (FAM and VIC) are as specified. For example, C__27102425_10AF is a modified C__27102425_10 assay allowing interrogation of rs16947 “A” with FAM. In the text we refer to the assays by their DropPhase2D6 assay name, which includes the SNP rs number and the allele of interest (generating a fluorescent signal). The SNP location indicates the location of rs16947 and rs3892097 within the CYP2D6 gene and the approximate downstream positions of SNPs interrogated for linkage with rs16947. NFQ, non-fluorescent quencher (or dark probe). Note that dbSNP reports rs5758562C>G (MAF=0.27) while we refer to “G” as reference and “C” as the variant.