Figure 2.

Tumor-free survival, tumor burden, and breast tumor histopathology are similar in NaHCO₃-treated mice and control mice. (A). Tumor-free survival of NaHCO₃-treated mice and control mice after carcinogen-based breast cancer induction. Median tumor-free survival of NaHCO₃-treated mice was 85.5 ± 8.2 days (n = 42) compared to 82.0 ± 7.5 days for control mice (n = 45). Data were compared by Gehan-Breslow-Wilcoxon test. (B). Tumor burden was similar in NaHCO₃-treated mice and control mice (n = 37–41) two weeks after first tumor detection. Tumors were typically 3–4 mm at first detection consistent with previous reports [49]. Data were log-transformed in order to improve normal distribution and then compared by unpaired two-tailed Student’s t-test. (C). Distribution of breast tumors from NaHCO₃-treated mice and control mice (n = 37–41) between histopathological subtypes. (D). Plot of matched data for histopathology, tumor burden, and tumor latency of breast tumors from NaHCO₃-treated mice and control mice (n = 37–41). NS: Not significantly different vs. Control.