Figure 3.

Metabolic pathways are dramatically perturbed during breast carcinogenesis but glycolytic metabolism in breast cancer tissue and normal breast tissue in vivo is unaffected by oral NaHCO₃ therapy. (A, B). Protein expression changes for metabolic pathways. We compare breast cancer tissue vs. normal breast tissue from control mice (A) and breast cancer tissue from NaHCO₃-treated mice vs. control mice (B). p-values were calculated based on Fisher’s right-tailed exact test. (C–E). Interstitial concentrations of glucose (C) and lactate (D) and corresponding [glucose]/[lactate]-ratios (E) measured in microdialysis samples from breast cancer tissue and matched normal breast tissue of NaHCO₃-treated mice and control mice (n = 8–9). Data were compared by repeated measures to-way ANOVA followed by Sidak’s post-tests. NS: not significantly different vs. Control. ***p < 0.001.