Figure 5.

NBCn1 and NHE1 protein expression is increased in organoids from breast cancer tissue compared to normal breast tissue, but unaffected by oral NaHCO₃ therapy. (A–C). Representative immunoblots (A) and quantified protein expression levels for NBCn1 (B) and NHE1 (C) in breast cancer tissue and normal breast tissue from NaHCO₃-treated mice and control mice (n = 11–12). Equal amounts of total protein were loaded in each lane of the gels. The band densities for NBCn1 and NHE1 are normalized to that of pan-actin. Data were compared by repeated measures two-way ANOVA followed by Bonferroni post-tests. **p < 0.01, ***p < 0.001. NS: not significantly different vs. Control. The Full uncropped images of immunoblots please find in Figure S4.