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. 2020 Apr 23;10(4):653. doi: 10.3390/biom10040653

Figure 2.

Figure 2

3-MST accumulates in the mitochondria of Down syndrome cells (DSC) compared to healthy control cells (CC) as shown by Western blotting. Top panels: chemiluminescence images of 3-MST protein expression in cytosolic (Cyto)- and mitochondrial (Mito)-enriched fractions of the selected healthy control fibroblast (Detroit 551; CC) and the Down syndrome fibroblast (Detroit 539; DSC). Experiments using four different cell passages are shown. The Western-blot analysis utilized β-actin as a loading control. The outer mitochondrial membrane transport protein Tom20 served as a control for the purity of the mitochondrial-enriched fractions. Bottom graph: statistical analysis of the healthy control fibroblast (Detroit 551; CC) and the Down syndrome fibroblast (Detroit 539; DSC). * p < 0.05.