Figure 3.

The 3-MST inhibitor HMPSNE suppresses H₂S levels in Down syndrome cells. Fluorescence values of the H₂S probe AzMC (A) and P3 (B) are shown in healthy control Detroit 551 fibroblasts (CC) and in Down syndrome Detroit 539 (DSC) fibroblasts under baseline conditions and when treated with 1, 3 or 10 µM HMPSNE for 24 h. Each bar represents the mean ± SEM of 4 independent experiments. *** p < 0.001 indicates a significant increase in fluorescence in DSCs vs. CCs; ^### p < 0.001 indicates a significant inhibitory effect of HMPSNE on cellular H₂S levels in DSCs.