Figure 5.

TIE2 expression reduces the proliferation of MCF-7 cells. (A) MCF-7 TIE2^tet and MCF-7 eGFP^tet cells were cultured in the presence or absence of doxycycline (Dox, 0.5-1 µg/mL). Proliferation was assessed by MTT. Values are represented as the average ± SEM. *** p < 0.001 vs. vehicle-treated cells using a two-way ANOVA with Bonferroni post-test. (B) Representative histograms of a cell cycle analysis of MCF-7 eGFP^tet and MCF-7 TIE2^tet cells cultured in the presence or absence of Dox (0.5–1 µg/mL) for 9 days. Percentages of non-proliferating (G₀/G₁) and proliferating (S + G₂/M) cells are indicated. Average percentages of cells in G₀/G₁, S, and G₂/M ± SEM of three independent cell cycle analyses. (C) Expression of MKI67, PCNA, CDKN1A, CDKN1B, and cyclin D1 (CCND1) in MCF-7 TIE2^tet or eGFP^tet cells cultured ±Dox (1 µg/mL) for 9 days. Results are represented as the average gene expression ±SEM vs. MCF-7 TIE2^tet vehicle-treated cells. * p < 0.05, ** p < 0.01, and *** p < 0.001 using a two-way ANOVA with Bonferroni post-test.