Figure 7.

TIE2 reduces the growth of orthotopic 4T1 TIE2^tet tumors. (A) 4T1 TIE2^tet cells were cultured in the presence or absence of doxycycline for 2 days before being inoculated bilaterally, in the 4th mammary fat pad of mice receiving or not doxycycline in their drinking water (n = 7 per group). (B) Water consumption and (C) tumor volume were measured throughout the experiment, and (D) the weight of excised tumors was measured at the time of the euthanasia. Results are represented as the average ± SEM. ** p < 0.01 and *** p < 0.001 vs. control mice using a two-way ANOVA with Bonferroni post-test (panels B and C). ^†† p < 0.01 using an unpaired Student’s t-test (panel D).