Figure 8.

TIE2 reduces osteolysis of 4T1 TIE2^tet bone metastasis. (A) 4T1 TIE2^tet cells were cultured in the presence or absence of doxycycline for 2 days before being inoculated in the left cardiac ventricle of mice receiving or not doxycycline in their drinking water (n = 7 per group). (B) Water consumption was measured throughout the experiment. Osteolytic lesions were assessed using (C) radiographs of the hind limbs of mice (white arrows indicate osteolysis area), and (D) the osteolysis area was measured. * p < 0.05 using a Mann–Whitney test. (E) Summary of the occurrence of osteolytic lesions on endpoint radiographs and of skeletal tumor burden on tissue sections. (F) The presence of skeletal tumor burden was assessed using tissue sections stained with hematoxylin, eosin, and orange G (representative sections). The yellow dotted lines contour and indicate the skeletal tumor burden.