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. 2020 Mar 30;12(4):827. doi: 10.3390/cancers12040827

Figure 5.

Figure 5

The combined effects of vorinostat with SASP are dependent on GSH and ROS. (A) Flow cytometry analysis of cell death induced by cotreatment of vorinostat with salazosulfapyridine (SASP) in the presence or absence of N-acetylcysteine (NAC). MDA-MB-231 and HCT116 cells were treated with 1.6 μM vorinostat, 500 μM SASP, or a combination of both with/without 5 mM NAC for 144 h. The percentages of cells in the sub-G1 population were analyzed by flow cytometry. Columns, means (n = 3); bars, SD. ** p < 0.01. (B,C) Colony formation of cells cotreated with vorinostat and SASP in the presence or absence of NAC. MDA-MB-231 and HCT116 cells were treated with a combination of 1.6 μM vorinostat and 500 μM SASP with/without 5 mM NAC for 144 h, and then the media were replaced. After the cells were cultured for 3–7 more days, the colonies were stained with crystal violet. Representative images of colony formation (n = 3) are shown (B). Colony formation was quantified using ImageJ (C). The data obtained with DMSO control were taken as 1. Columns, means (n = 3); bars, SD. ** p < 0.01.