FIGURE 2.
BMP8A promoted clear cell renal cell carcinoma (ccRCC) proliferation and chemoresistance and inhibited apoptosis. A, BMP8A expression levels in the RCC cell lines compared with that in the human renal tubular epithelial cell line. B, Detection of BMP8A expression in 7 cell lines by ELISA. C, The tumor sections were subjected to IHC staining using antibodies against BMP8A and p‐SMAD1/5/9. D, Using CCK‐8 assays to detect cell proliferation after different treatments. E, CCK‐8 assays to detect the effects of different concentrations of BMP8A on cell proliferation. F, Detection of si‐BMP8A/si‐Ctl clone formation assays. G, Hoechst staining assay performed in ACHN and 786‐O cells with si‐BMP8A/si‐Ctl. H, The IC50 values of As2O3 were determined by CCK‐8 assay in 786‐O cells. I, Apoptosis levels revealed by casp.3/7 activity assay followed with the indicated treatments in 786‐O and ACHN cells. The data represent the mean ± SD of three replicates. *P < 0.05, **P < 0.01, *** P < 0.001