FIGURE 3.

BMP8A promoted the activation of Nrf2 in clear cell renal cell carcinoma (ccRCC). A, Effect of BMP8A on ARE transcription activity detected by ARE reporter assay. B, Correlation of expression between BMP8A and Nrf2 in 540 ccRCC samples from The Cancer Genome Atlas (TCGA) dataset. C and D, Nrf2 mRNA levels were analyzed after BMP8A stimulation/KD. E‐I, Western blot analysis of the treated cell samples. J, In the si‐Ctl and si‐BMP8A cells in the presence of 100 mg/mL CHX for the indicated time periods. Nrf2 levels in the si‐Ctl cells were normalized to 1. K and L, the interaction between P62, Keap1 and Nrf2/(Ub‐Nrf2) was determined by a co–immunoprecipitation (co‐IP) assay. M, Relative amounts of Nrf2 in the nuclear or cytoplasmic fractions were determined by a cell fraction assay after BMP8A or si‐BMP8A. The data represent the mean ± SD of three replicates. n.s. not significant