FIGURE 4.

Nrf2 regulated transcription of TRIM24 in clear cell renal cell carcinoma (ccRCC). A, GSEA analysis revealed that the bone morphogenetic proteins (BMP) signaling pathway was significantly altered in ccRCC. B, TRIM24 mRNA levels were analyzed after Nrf2 KD. C‐E, H, Western blot analysis was performed to assess the expression of TRIM24 with the indicated treatments. F‐G, I, TRIM24 mRNA levels were analyzed with the indicated treatments. J, 786‐O cells were subjected to ChIP assay using Nrf2 antibody, followed by qRT‐PCR analysis using primers targeting the promoter regions of TRIM24 (NQO1 was used as a positive control and p21 was used as a negative control). K, Detecting casp.3/7 activity with the indicated treatments. L, CCK‐8 assays performed with the indicated treatments. M, Tumor sections were subjected to IHC staining using antibodies against BMP8A TRIM24, p‐Nrf2 and Nrf2. *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001