FIGURE 7.
BMP8A/Nrf2/TRIM24‐induced reactive oxygen species (ROS) inhibition and Wnt pathway activation correlated with As2O3 resistance. A, ROS levels as indicated by DCFH‐DA fluorescence. B, The average cell death rates in 786‐O and ACHN. C‐G, TRIM24, Nrf2, Bax, Bcl2, cleaved caspase‐3, Wnt pathway and EMT‐related proteins were determined by western blotting. H‐J, Images as well as tumor weight at day 35 (H) and tumor volumes at the indicated dates (I). J, Represents the weight of the tumor. K, L, TRIM24, Nrf2, p‐Nrf2, Bax, Bcl2 and cleaved caspase‐3 were determined by western blotting and antioxidative Nrf2 targets were measured by real‐time RT‐PCR in samples derived from ACHN xenografts. *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001