Silencing for Kv10.1 results in an overexpression of proteins involved in fussion processes. (a) representative images of Western Blot of proteins involved in mitochondrial dynamics in DU145, DU145KD, HeLa and HeLa KD cells. Actin was used as a loading control in all cells. The bands correspond to t = 72 h of the same blot for each cell line, except for MFN-2, which is a different membrane, and therefore has a different actin loading control. (b,c) Densitometric analysis of the protein content in DU145, Du145KD (b), HeLa and HeLa KD cells (c); the values were normalized to the loading control. Bars represent the mean of the three independent experiments. Error bars indicates SEM (* p ˂ 0.05, ** p ˂ 0.001, **** p < 0.0001, 2-waz ANOVA).