Figure 7.

Identification of cardiolipin species that activates type 1 NKT cells. L-CD1d cells were pulsed in the presence of either purified (A) 18:1 or (B) 16:1 cardiolipin for 4 h at 37 °C prior to co-culture with DN32.D3 for 24 h. IL-2 production was then measured by ELISA. Each ELISA was conducted in triplicate. Structures of 18:1 and 16:1 cardiolipin are shown in (C), (D) respectively with the head group in blue, the acyl chain in black, and the extra carbons in red. Data was analyzed using a student’s t-test. * p < 0.05; ** p < 0.005.