Table 1.
Sample and dilution volumes with cell counts processed by CellSearch and ISET for CTC enumeration.
| Sample Characteristics | Unit/Blood Cells | CellSearch (n = 16) | ISET (n = 16) |
|---|---|---|---|
| Sample volume | DLA product (mL) | 1.5 (1.1–2.5) | 10 |
| Absolute number of processed blood cells (× 108) | Leukocytes | 2 | 10 (7.1–15.9) |
| Lymphocytes | 0.8 (0.6–1.1) | 4.3 (3.7–6.9)) | |
| Monocytes | 0.4 (0.3–0.5) | 2.1 (1.3–3.7)) | |
| Granulocytes | 0.9 (0.7–1.1) | 5.3 (2.1–7.6) | |
| Platelets | 26.3 (19.3–44.7) | 152.6 (91.4–172.7) | |
| Erythrocytes | 9.6 (5.6–1.4) | 65.0 (45.5–91.8) | |
| Dilution and total volumes | Total sample (mL) | 7.5 | 110 |
| Dilution material | CellSearch buffer | ACDA/ISET buffer | |
| Dilution volume | 6 (5.0–6.4) | 10/90 | |
| Concentrations per mL sample (× 106/mL) | Leukocytes | 26.7 | 9.0 (6.4–14.5) |
| Lymphocytes | 10.4 (8.1–14.4) | 4.0 (3.4–6.2) | |
| Monocytes | 5.7 (4.4–6.5) | 1.9 (1.2–3.4) | |
| Granulocytes | 12.6 (9.5–14.3) | 4.8 (1.9–6.9) | |
| Platelets | 350.6 (257.2–596.5) | 138.7 (83.1–157.0) | |
| Erythrocytes | 0.1 (0.1–0.2) | 0.1 (0.1–0.1) | |
| Limiting factor | Number of leukocytes | NA |