Table A1.
Characteristics of filtered diagnostic leukapheresis samples by ISET®, according to original protocol (cohort 1), adjusted protocol (cohort 2) and the live cell protocol by half the filter.
| Processed | Blood Cells | ISET Cohort 1 (n = 18) * | ISET Cohort 2 (n = 16) ** | ISET Live Cell (n = 8) *** |
|---|---|---|---|---|
| Sample volume (mL) | DLA product | 0.5–5 | 5 | 10–20 |
| Absolute blood cell counts processed (×108) | Leukocytes | 4.6 (3.7–7.3) | 5 (3.6–8.0) | 18.6 (9.6–33.9) |
| Lymphocytes | 1.9 (1.5–2.7) | 2.2 (1.9–3.5) | 7.7 (4.8–12.0) | |
| Monocytes | 0.8 (0.6–1.2) | 1.1 (0.7–1.8)) | 2.4 (1.6–4.7) | |
| Granulocytes | 1.9 (0.9–3.5) | 2.6 (1.1–3.8) | 8 (3.3–17.5) | |
| Platelets | 78.7 (69.5–94.8) | 76.3 (45.7–63.9) | 319.4 (199.1–500.8) | |
| Erythrocytes | 19.8 (15.7–32.1) | 32.5 (22.8–45.9) | 76.5 (50.5–219.0) | |
| Dilution and total processed sample (mL) | Dilution material | DPBS and fixed ISET buffer | ACDA and fixed ISET buffer | ACDA and live ISET buffer |
| Dilution volume | 0–4.5 and 45 | 5 and 45 | 10–20 and 80–160 | |
| Total sample | 50 | 55 | 100–200 | |
| Concentrations per mL sample (×106/mL) | Leukocytes | 9.2 (7.4–14.6) | 9.0 (6.4–14.5) | 9.3 (7.6–26.2) |
| Lymphocytes | 3.8 (2.9–5.5) | 4.0 (3.4–6.2) | 4.1 (3.9–7.8) | |
| Monocytes | 1.6 (1.3–2.4) | 1.9 (1.2–3.4) | 1.4 (1.2–3.5) | |
| Granulocytes | 3.8 (1.9–7.0) | 4.8 (1.9–6.9) | 4.0 (2.5–15.0) | |
| Platelets | 157.3 (139.1–189.6) | 138.7 (83.1–157.0) | 268.9 (142.7–314.5) | |
| Erythrocytes | 0.04 (0.03–0.06) | 0.1 (0.1–0.1) | 0.1 (0.1–0.1) | |
| Limiting factor | Platelets | None | None |
* Description of material filtrated by the large compartment of the ISET® filtration block is shown. ** Total filtered volume has been divided by two, for comparison with the other two protocols. *** Description of the material filtrated by the small compartment of the ISET® filtration block is shown.