Table 1.
Data collection and refinement statistics.
| Data Collection | |||||
|---|---|---|---|---|---|
| Beamline | PX1, SOLEIL | ||||
| Wavelength (Å) | 0.97918 | ||||
| Space group | P1 | ||||
| a, b, c (Å) | 57.57, 64.47, 67.59 | ||||
| α, β, γ (°) | 96.35, 101.95, 97.27 | ||||
| No. of monomers in asymmetric unit | 4 | ||||
| Resolution (Å) | 32.73–1.9 (1.94–1.9) | ||||
| Total no. of reflections | 451,693 (25,605) | ||||
| No. of unique reflections | 73,400 (4491) | ||||
| Completeness (%) | 99.7 (98.8) | ||||
| Multiplicity | 6.2 (5.7) | ||||
| Mean I/σ (I) | 11 (2.8) | ||||
| Rmerge | 0.073 (0.529) | ||||
| Rmeas | 0.088 (0.649) | ||||
| CC1/2 | 0.997 (0.902) | ||||
| Wilson B factor (A°2) | 23.8 | ||||
| Refinement | |||||
| Resolution (Å) | 32.73–1.90 | ||||
| No. of reflections | 73,390 | ||||
| No. of reflections in test set | 3643 | ||||
| Rwork/Rfree | 0.169/0.213 | ||||
| Rmsd bonds (Å) | 0.014 | ||||
| Rmsd angles (°) | 1.846 | ||||
| Rmsd Chiral (Å3) | 0.091 | ||||
| No. Atoms/Bfac (Å2) | Chain A | Chain B | Chain C | Chain D | |
| Protein | 1805/30.1 | 1779/30.7 | 1805/30.7 | 1747/31.7 | |
| Glycan | 94/40.7 | 28/51.8 | 28/58.0 | 72/47.9 | |
| Metal ions | 2/22.7 | 2/23.0 | 2/22.4 | 2/23.0 | |
| Water | 129/37.1 | 131/36.5 | 136/37.0 | 124/36.5 | |
| Ramachandran Favored (%) Allowed (%) Outliers (%) |
97 3 0 |
||||
| PDB Code | 6TT9 | ||||
Note: Values in parentheses are for the outer shell. PDB: Protein data bank.