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Cytoskeleton remodeling under NP treatment. (a) IXplore SpinSR Olympus super-resolution microscopy of F-actin staining (green). Cells were treated for 24 h with nanoparticles 50 μg Fe mL−1, fixed and stained for F-actin. Binarization was done using ImageJ software. (b) Cells were treated with NPs as in a. After the treatment cells were fixed and stained for tubulin (red). Super-resolution imaging was done using IXplore SpinSR Olympus super-resolution system.
