Figure 1.
Molecular analysis of pooled venom from Australian scorpion Hormurus waigiensis. (A) Image of a scorpion on the pad used for venom collection by electrical stimulation of the telson. (B) The crude venom chromatogram at A280nm from SE-FPLC highlighting the 12 resolved peaks (designated as peak samples 1–12b); venom Pools (A–F) used for the bio-impedance study are shown in relation to the corresponding peak samples and the elution fractions. (C) An example of the LCMS analysis for the second elution fraction (EF 20) within peak sample 5, where 41 molecular weights were identified. The inset mass spectrum illustrates an example of the underlying mass spectra of the highlighted (red) LCMS chromatogram peak and the corresponding molecular weight of the molecule. (D) A box plot analysis of the molecular weight distributions of all 14 peak samples (see also Table S1). The boundaries of the box plots represent 25% and 75% percentiles, the solid line is the median, the dashed line is the mean, 95% percentiles are also shown; individual data points are overlaid. The inset shows the number of distinct molecules identified within each peak sample. (E) Size-ranked plot of the molecular weights of the 182 molecules identified in the venom fractions.