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. 2020 Apr 23;9(4):1057. doi: 10.3390/cells9041057

Figure 4.

Figure 4

CPZ affects both clathrin and AQP2 subcellular distribution in rat kidney slices. Rat kidney slices were incubated with or without CPZ (400 µM for 15 min), and then, AQP2 and clathrin were immunolocalized. To test cell viability, some slices were treated with vasopressin (AVP, 100 nM for 10 min). After AVP treatment, the apical AQP2 signal was greatly increased in principal cells, with increased apical clathrin signals also (middle panels). In contrast, after CPZ treatment, the basolateral AQP2 signal was markedly increased along with the basolateral clathrin signal (lower panels).