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. 2020 Apr 10;10(4):585. doi: 10.3390/biom10040585

Figure 4.

Figure 4

Deletion of VDAC1 exacerbates ischemia-induced interstitial collagen accumulation after acute injury. Kidneys were harvested before (sham) and on day 7 after ischemia (I/R) to assess collagen accumulation in the tissue. Formalin-fixed and paraffin-embedded 4–8-μm tissue sections were stained with picro-sirius red. Collagen staining in the renal interstitium was assessed by light microscopy using circularly polarized light. The images were captured using Nikon Eclipse E800 microscope using Nikon 10× Plan Apo objective. Magnification: 122×. (A) Representative kidney sections from three wild-type (WT) sham mice. (B) Representative kidney sections from four wild-type (WT) mice on day 7 after ischemia. (C) Representative kidney sections from three sham VDAC1-deficient (KO) mice. (D) Representative kidney sections from four VDAC1-deficient (KO) mice on day 7 after ischemia. The arrows mark sites with increased collagen accumulation.