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. 2020 Apr 20;9(4):1020. doi: 10.3390/cells9041020

Figure 6.

Figure 6

MALAT1 depletion impedes xenograft growth of HCC cells. Luciferase-expressing SK-Hep1 cells with or without MALAT1 depletion were injected into NOD/SCID mice. (A) Representative images comparing the tumorigenic potential in NOD/SCID mice bearing scrambled control or shMALAT1#2 SK-Hep1 tumors over a period of 5 weeks using bioluminescence imaging technique. Semi-quantitative analysis of the change in bioluminescent intensity (fold change) over time curve showed a significantly lower tumor burden was in the shMALAT1 group. (B) Photographs of the tumor samples harvested, and tumor volumes measured at week 5, demonstrating the shMALAT1 tumors were significantly smaller than the control counterparts. (C) Representative histochemical staining of Ki-67 and PCNA in primary HCC tumor cells from control and MALAT1 shRNA-transfected mice. The percentage of tumor cells that were positive for Ki-67 and PCNA was calculated by counting 10 visual fields at high magnification. Scale bars, 50 μm. Bioluminescence intensity, BLI; PCNA, proliferating cell nuclear antigen. Data are mean ± SEM **, p < 0.01; ***, p < 0.001.