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. 2020 Apr 18;10(4):629. doi: 10.3390/biom10040629

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of two kinds of mitochondrial membrane-anchored AAA+ proteases. (a) Cartoon representation of i- and m-AAA proteases anchored in the mitochondrial inner membrane (IM) with opposite orientations to intermembrane space (IMS) and matrix space, respectively. (b) The pore loop spiral staircases surrounding the substrate in yeast Yme1 (PDB ID: 6AZ0; left) and human AFG3L2 (PDB ID: 6NYY; right). Aromatic residues in pore loops are all highlighted as sticks and spheres, which in pore-2 loops are colored gray.