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. 2020 Apr 21;9(4):1027. doi: 10.3390/cells9041027

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Active matriptase, but not inactive S805A or disease-associated G827R mutant matriptase, promotes cleavage of EpCAM and TROP2. 293T cells were transfected using Fugene 6 with fixed amounts of pcDNA3 encoding HA-TROP2 and varying amounts of Flag-matriptase expression plasmid (A), pcDNA3 encoding HA-EpCAM and plasmid encoding Flag-matriptase, Flag-matriptase S805A, or Flag-matriptase G827R (B), pcDNA3 encoding HA-TROP2 and plasmids encoding Flag-matriptase or mutants (C). 48 h after transfection cell lysates were prepared and resolved, and the indicated epitope-tagged proteins were analyzed via Western blotting using anti-HA and anti-Flag. Representative data from 1 of 3 experiments is shown.