Table 1.
Parameters characterizing the immobilization process (amount of the immobilized enzyme, immobilization yield, specific activity and relative activity of free and immobilized laccase, and kinetic parameters of the free and immobilized enzyme.
| Analyzed Parameter | Free Laccase | Immobilized Laccase |
|---|---|---|
| Immobilization yield (%) | - | 91 ± 0.6 |
| Amount of immobilized enzyme (mg/g) | - | 1820 ± 90 |
| Specific activity (U/mg) | 56 ± 1.9 | 52.5 ± 1.5 |
| Activity retention (%) | - | 89 ± 3.2 |
| KM (mM) | 0.093 ± 0.018 | 0.113 ± 0.022 |
| Vmax (mM/s) | 0.048 ± 0.008 | 0.045 ± 0.011 |
| Vmax/KM (1/s) | 0.516 | 0.398 |