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. 2020 Mar 27;10(4):511. doi: 10.3390/biom10040511

Figure 6.

Figure 6

Octominin inhibits the LPS-induced inflammation in macrophages via blocking TLR4/NF-κB signal transduction. The gene expression analysis of TLR2 (a) and TLR4 (b) was measured using qPCR with GAPDH as the internal reference gene. The 2−ΔΔCt method was used to calculate the relative mRNA expression levels. The translocation of NF-κB p50 and p65 to the nucleus was analyzed by Western blot analysis of cytoplasmic (c) and nuclear (d) protein extracts. Equal protein loading was controlled using antibodies against β-actin and nucleolin in cytosolic and nuclear extracts, respectively. Relative expression of the bands was analyzed using ImageJ software (e,f). Results are expressed as the mean ± SD of three separate experiments. Statistical significance was determined versus vehicle-treated control group (# <0.05 and ## <0.01) or LPS-treated group (* <0.05 and ** <0.01).