Table 1.
Reagent/Chemical/Antibody | Manufacturer | Reference |
---|---|---|
Neurobasal medium | Gibco | 21103049 |
B27 | Gibco | 17504-044 |
GlutaMAX | Gibco | 35050-038 |
Tripanblue solution | Sigma | T8154 |
Chemilluminescence ECL kit | Santa Cruz Biotechnology | sc-2048 |
Normal donkey serum | Jackson Immunores. Labs. | 017-000-121 |
4% paraformaldehyde | Pierce | 28908 |
Anti-GSK3β | Cell Signalling | #9315 |
Anti-phosphoGSK3βat Ser9 | Cell Signalling | #9336 |
Anti-MAP2 | Sigma-Aldrich | M1406 |
Anti-α-Tubulin | Sigma-Aldrich | T9026 |
Horseradish peroxidase-conjugated secondary antibody | Promega | W4021 |
Alexa fluor 488-conjugatedanti-mouse | Jackson Immunores. Labs. | 715-545-150 |
DAPI Fluoromont | Southern Biotech | 010020 |