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. 2020 Mar 26;9(4):806. doi: 10.3390/cells9040806

Figure 5.

Figure 5

Effect of GAL1 on MMPs and EMT pathway proteins assessed through Western blotting analysis. Mock: cells treated with DMSO vehicle only. β-Actin was used as the protein loading control. (A) Total cell lysates of the BFTC-909, T24, and J82 cells treated with shRNA or GAL1 recombinant protein (1 μg/mL) were analyzed in terms of expression levels of MMP-2, MMP-9, and TIMP-1 by Western blotting. (B) Total cell lysates of the BFTC-909, T24, and J82 cells treated with shRNA or GAL1 recombinant proteins (1μg/mL) were analyzed in terms of expression levels of EMT pathway through Western blotting analysis.