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Generation of the anti-CSPG4-IgG1 antibody. (A) Schematic diagram summarizing the development of stable cell lines expressing anti-CPSG4 IgG. Vectors were linearized before transfection to allow correct integration into the host genome, and transgene-expressing cells were selected; (B) pVITRO1-CSPG4-IgG1/κ vector map; (C) UCOE-CSPG4-HC(γ1) and UCOE-CSPG4-LC (κ) vector maps.
