Figure 1.

KRAS^G12V and KRAS^G12D mutations do not affect cell proliferation. (a) Immunofluorescence (IF) staining of F-actin in H838 KRAS isogenic cell lines. F-actin is visualized by fluorescent green phalloidin-staining Acti-stain™ 488. DAPI is the blue nuclear stain. Immunoreactivity was captured by confocal microscopy. (b) The cell proliferation of the H838 KRAS isogenic cell lines are the same. Cell proliferation was measured using a CellTiter-Glo Luminescent Cell Viability assay. (c) The ability of colony formation in H838 KRAS isogenic cell lines. The cell colonies were stained with crystal violet and counted 7 days after cell seeding. The values represent the means ± s.d. of three independent assays (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001).