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. 2020 Mar 31;12(4):837. doi: 10.3390/cancers12040837

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The inactivation of RhoA restores the activity of Wnt/β-catenin pathway. (a) The activity of Wnt/β-catenin in H838KRAS isogenic cells as measured by TCF/LEF reporter assays. (b) The western blotting of Wnt/β-catenin pathway-related genes. β-actin was used as an internal control. (c) The activity of Wnt/β-catenin after inactivation of RhoA by transfecting with RhoA^T19N plasmids in H838KRAS^G12D cells. (d) The activity of Wnt/β-catenin after inactivation of RhoA by treating with Y27632 inhibitor in H838KRAS^G12D cells. The activity of Wnt/β-catenin were access by TCF/LEF reporter assays. All reporter activities were evaluated by a dual luciferase system, and the values represent the means ± s.d. of six independent assays (n = 6, * p < 0.05 and ** p < 0.01). The uncropped blots and molecular weight markers are shown in Figure S10.