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. 2020 Apr 23;9(4):1053. doi: 10.3390/cells9041053

Figure 2.

Figure 2

Lactate induced SIRT1 downregulation, contributing to normal kidney cell line pseudo-transformation. Characterization of SIRT1 expression in normal kidney cell line treated with 20 mM lactate by RT-qPCR (A), Western blot (B), and immunofluorescence (C). Characterization of global histone acetylation of histone H3 and specific histone marker H3K9 in normal kidney cell lines treated with 20 mM lactate by Western blot (D). Effect of 20 mM lactate treatment in cell proliferation (BrdU assay) (E), cell migration (wound-healing assay) (F), and cell invasion (Matrigel Invasion Chambers) (G). Western blot and immunofluorescence quantification are represented as fold change of 20 mM lactate versus control condition; * p < 0.05, **** p < 0.0001, and ns p > 0.05 (non-significant). Abbreviations: C/CTR—control, L/LAC—20 mM lactate.