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. 2020 Apr 23;9(4):1053. doi: 10.3390/cells9041053

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Nicotinamide (NAM) mimicked lactate effect in kidney cell lines. Characterization of SIRT1 expression by RT-qPCR (A), Western blot (B), and immunofluorescence (C). Characterization of global histone acetylation of histone H3 and specific histone marker H3K9 (D) by Western blot. Effect at cell proliferation by BrdU assay (E), cell migration by wound-healing assay (F), and cell invasion by Matrigel invasion chambers (G). Western blot and immunofluorescence quantification are represented as fold change of 200 µM nicotinamide versus control condition; * p < 0.05, *** p < 0.001, **** p < 0.0001 and ^ns p > 0.05 (non-significant) Abbreviations: C/CTR—control, N/NAM—200 µM nicotinamide.