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. 2020 Apr 23;9(4):1053. doi: 10.3390/cells9041053

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Alpha-cyano-4-hydroxycinnamate (CHC) reverted the lactate effect in normal kidney cell lines. Characterization of SIRT1 expression by RT-qPCR (A,G) and Western blot (B,H). Characterization of global histone acetylation of histone H3 and specific histone marker H3K9 (C,I) by Western blot. Effect at cell proliferation (D,J) by BrdU assay and cell migration (E,K) by wound-healing assay. Measurement of lactate levels after CHC treatment in RCC cell lines (F); * p < 0.05, ** p < 0.01, *** p < 0.001, , **** p < 0.0001 and ^ns p > 0.05 (non-significant) Abbreviations: CTR—control, CHC—alpha-cyano-4-hydroxycinnamate, CM—conditioned medium, LAC—20 mM lactate.