Table 1.

Oligonucleotides used in this study.

Gene	Forward primer	Reverse primer	Tan	Cycles
ARID1A-5″	TCTTGCCCATCTGATCCATT	CCAACAAAGGAGCCACCAC	60 °C	40
ARID1A-flank. guideRNA A,B	CGCAGCAAGGACATGGGTA	ATGGAGTCTGGCCCTGTTGA	60 °C	40
ARID1A-flank. guideRNA C	TCTCAGCAGTCCCAGCAAAC	AGGCAAGCTGGAGGGTCTT	60 °C	40
ARID1B	CAAGGGGATCAGAGCAACCC	CTACCTGGGATACTTGCAGGA	60 °C	45
ATF3	AAGAACGAGAAGCAGCATTTGAT	TTCTGAGCCCGGACAATACAC	60 °C	40
ATM	TGGATCCAGCTATTTGGTTTGA	CCAAGTATGTAACCAACAATAGAAGAAGTAG	60 °C	40
ATR	CAGCTTTGTGCCATTTACTG	CTACCTCAATTCCAAGCACA	60 °C	40
CDKN1A	CCTCATCCCGTGTTCTCCTTT	GTACCACCCAGCGGACAAGT	60 °C	40
CHEK1	ATATGAAGCGTGCCGTAGACT	TGCCTATGTCTGGCTCTATTCTG	60 °C	40
DUSP1	GTACATCAAGTCCATCTGAC	GGTTCTTCTAGGAGTAGACA	60 °C	40
ETAA1	GAGAATTTCCATACATTTCCCCTTT	CTAAACAAGGAAGTAATTTGGTACAATCAA	60 °C	40
FGF4	TTCTTCGGGCCATGAGCAG	CCGAAGAAAGTGCACCAAGG	60 °C	40
FOS	GAGAGCTGGTAGTTAGTAGCATGTTGA	AATTCCAATAATGAACCCAATAGATTAGTTA	60 °C	45
GADD45B	GTCGGCCAAGTTGATGAAT	CACGATGTTGATGTCGTTGT	60 °C	40
GAPDH	TGCCAAATATGATGACATCAAGAA	GGAGTGGGTGTCGCTGTTG	60 °C	45
ID2	TCAGCCTGCATCACCAGAGA	CTGCAAGGACAGGATGCTGATA	60 °C	40
MLH1	CTTGTACCCCCCGGAGAAG	TGCAACATCTCCCGGAGAAC	60 °C	40
P53	TTGCAATAGGTGTGCGTCAGA	AGTGCAGGCCAACTTGTTCAG	60 °C	40
SMARCA4	CAGCATGCCAAGGATTTCAAG	CGATCCGCTCGTTCTCTTTC	60 °C	40
SMARCB1	AACGTCAGCGGGTTCAAAT	GCCTTCACCTGGAACATGAA	60 °C	40
TOP2A	AGTCATTCCACGAATAACCA	TTCACACCATCTTCTTGAG	60 °C	40
TOPBP1	TGTGACTGGCTTATGTGGCT	TGGCACACTCATACTTCTGACC	60 °C	40
TP53BP1	ATTGAGGATACGGAACCCATGT	TGCTGGATTCATCAGGATACTATCA	60 °C	40
XPC	GGCCAAAGGTCTGCTCATCA	GTCCACCTCCTGCATCTGTG	60 °C	40
ZMYND11	TTGTTAAACGTGCCATGACC	GCATGTGTGGAGACAGAGGA	60 °C	40
ARID1A genotyping	GCGGTACCCGATGACCATGC	TACTGGAGGTCATTGAGGGG	60 °C	45
ARID1A guideRNA A	GCGGTACCCGATGACCATGC
ARID1A guideRNA B	ATGGTCATCGGGTACCGCTG
ARID1A guideRNA C	CCCCTCAATGACCTCCAGTA