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Image processing workflow to quantify the hybrid epithelial/mesenchymal (E/M) score. (a) Input images, (b) image registration and automatic annotation of the pan-CK-positive (pan-CK+) regions at low (4×) magnification, (c) manual exclusion of irrelevant annotations (e.g., necrosis areas and/or tissue defects), (d) Vim-positive (VIM+) and pan-CK+ detection in the final annotations (cf. details in Figure 3), and (e) E/M score computation (cf. details in Figure 3).
