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. 2020 Apr 8;12(4):906. doi: 10.3390/cancers12040906

Figure 3.

Figure 3

Steps of the hybrid E/M score computation. Registered images (from the same tissue slide) processed at 10× magnification (0.906 µm-side pixel): (a,b) VIM and pan-CK expression, respectively, on registered image areas; (c,d) detection of cell nuclei (blue), VIM+ (red) and pan-CK+ (green) areas; and (e) detection of pan-CK+/VIM+ area (yellow), pan-CK+/VIM− area (green), pan-CK-/VIM+ area (red). The hybrid E/M score was computed as the ratio between the yellow area and the sum of the yellow and green (i.e., pan-CK+) areas.